Yokogawa CV1000™Applications and Images

Neuronal Monitoring

  • Chicken cerebral cortex
  • To observe elevator activity (interkinetic nuclear movement by time-lapse imaging) in mouse neurons
  • FP: GFP
  • Time lapse interval 30 min., up to 40 hours, 5 fields, Z thickness 40 (11 slices at 4 interval)
  • EX: 488nm
  • Objective lens: 20X 0il

Neuronal Monitoring

  • Brain slice of mouse fetus
  • To observe particle movement
  • FP: GFP, Strawberry (position marker)
  • EX: 488nm 10%, 561nm 12%
  • EMCCD exposure and gain: 488nm 300ms, 100%, 561nm 200ms, 100%
  • Objective lens: 60X oil
  • Conditions: time lapse, 1 min. interval up to 5 hours, 1 field
  • Z thickness 30 (61 slices at 0.5 interval)