Yokogawa CV1000™Applications and Images

High-speed 4D imaging

  • Green: Membrane (EGFP)

Floating 293F cells were transfected with eGFP by using NeoFection, a transfection accelerating agent made by ASTEC.The cells were shake-cultured over night. As a result of time lapse imaging, active movement of cells expressing eGFP inside the floating cell clusters, and structural changes in the cell wall such as ruffing were clearly observed.

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Imaging Condition
Total Time 20 Hours
Interval 10 minutes
Z-Sections/Stack 101 Sections
(0.3um apart)
Imaging Positions 25 fields
Excitation 488nm
Objective Lens 60x Oil