Crest CARVII™Applications and Images

Fluorescence Recovery After Photobleaching (FRAP)

Our FRAP capability allows software control of a variable rectangular aperture for photobleaching a selected area with full spectrum (white) light. A software protocol can then be set up to record fluorescence before and after bleaching. The recovery kinetics can be analyzed using a variety of commercially available software packages.

Time lapse images and FRAP kinetics of endoplasmic reticulum in a plant cell stained with the membrane dye 3,3-dihexyloxacarbocyanine iodide (DiO6(3), Molecular Probes, Eugene, Oregon). The FRAP protocol including image capture and analysis was performed using Crest IPLab™ imaging software.

Dual emission imaging (FRET)

The Crest CARV II imager, in combination with the Dual View™ adapter from Photometrics, Tucson, AZ, allows you to perform dual emission imaging including CFP/YFP and GFP/RFP Fluorescence Resonance Energy Transfer (FRET).