Crest CARVII™Applications and Images

High speed calcium imaging

The Crest CARV II™ imager, in combination with CCD cameras with on-chip amplification, can be used to image fast fluorescence changes at rates ranging from 50–100 frames per second.

Time lapse recordings at 50 frames per second of calcium sparks in muscle cells loaded with calcium indicator dye Fluo-4, Molecular Probes, Eugene, Oregon. The Roper Cascade 512B CCD camera in conjunction with Metamorph® (Molecular Devices, Sunnyvale, CA) imaging software was used to capture the images.